First order derivative spectroscopy method involves the measurement of absorbances at zero crossing point of other drug. A newsimple, accurate, economic and sensitive first order derivative spectrophotometric method is proposed for the simultaneous estimation of Sildenafil Citrate and Dapoxetine Hydrochloride in their combined dosage form. Due to mutual interference, quantitation was carried out by the proposed methods namely first order derivative spectroscopy. The drugs obeyed the Beer’s law in the concentration range of 5-30μg//ml (r2 = 0.9984 and r2 = 0.9992) for Sildenafil Citrate and Dapoxetine Hydrochloride both. In the proposed method, absorbance was measured at 265.8 nm (Zero Crossing Point of Sildenafil Citrate) and 250.3 nm (Zero Crossing Point of Dapoxetine Hydrochloride). The percent recoveries were found near to 100% for both the drugs indicated that method is precise and reproducible. LOD for Sildenafil Citrate and Dapoxetine Hydrochloride was found to be 0.566 μg/ml and 1.65 μg/ respectively. LOQ was found to be 1.71 μg/ml and 5.0 μg/ml respectively.
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